FIELD: medicine.
SUBSTANCE: seeds of transgenic plants Nicotiana tabacum L. are sterilised, removed on hormone-free feeding medium and germinated in virto on light for 28 days. Obtained acrospires are cut into segments with size 0.2-0.3 cm2, removed onto modified feeding medium, which includes 2,4-dichlorophenoxyacetic acid, indolylacetic acid and kinetin. For seed sterilising solution which consists of 96% ethanol, 37% hydrogen peroxide and water in volume ratio 7.5:1:1 is used. Formed callus which contains gene of human interleukin-18 is cultivated in darkness for 21-28 days and multiplied by passing to required quantity.
EFFECT: invention can be used in pharmacology for growing biomass of cellular culture of transgenic tobacco Nicotiana tabacum L.
4 cl, 3 tbl, 1 ex
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