CARRIER FOR AFFINE CHROMATOGRAPHY AND METHOD OF PURIFYING BIOLOGICAL SUBSTANCE Russian patent published in 2019 - IPC G01N30/88 

FIELD: instrument making.

SUBSTANCE: invention relates to a carrier for affine chromatography and a method of purifying a biological substance using an affinity chromatography carrier. Carrier for affine chromatography comprises: a substrate; hydrophilic polymer; and an affinity ligand, in which the support consists of at least one selected from a group consisting of a polysaccharide, an acrylate-based polymer, a methacrylate-based polymer and a styrene-based polymer, hydrophilic polymer is at least one selected from a group consisting of dextran, carboxymethyldextran and pullulan, the affinity ligand is at least one selected from a group consisting of an antibody-binding protein and an antibody-binding polypeptide, carboxy group is introduced into carrier for affine chromatography and amount of introduced carboxy group ranges from 15 mmol/l of gel to 60 mmol/l of gel in relation to ion-exchange capacity.

EFFECT: development of carrier for affine chromatography with high capacity of adsorption of antibodies and high degree of purification.

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