FIELD: purifying diglycosylated interferon-beta protein.
SUBSTANCE: method for purifying diglycosylated interferon-beta protein, according to which (a) a culture solution containing interferon-beta expressed in a host cell is obtained; (b) affinity chromatography on the culture solution obtained in step (a) is performed; (c) solution obtained in step (b) is subjected to low pH inactivation wherein said low pH is pH 4.0 or less; (d) hydrophobic interaction chromatography is carried out on the solution obtained in step (c); (e) anion exchange chromatography is carried out on the solution obtained in step (d); and (f) cation exchange chromatography is performed on the solution obtained in step (e).
EFFECT: ensuring sufficient protein and high purity and reduction of the occurrence of glycosylation modification or other interferon-beta modifications.
9 cl, 2 dwg, 3 tbl, 2 ex
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