FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology and describes a new bacterial nuclease of the CRISPR-Cas9 system from the bacterium Capnocytophaga ochracea, as well as an application thereof for forming strictly specific double-stranded breaks in the DNA molecule. The nuclease herein has unusual properties and can be used as a tool for introducing alterations at strictly defined places in the sequence of genomic DNA of unicellular or multicellular organisms.
EFFECT: invention increases the versatility of available CRISPR-Cas9 systems, thereby allowing for the use of Cas9 nucleases from various organisms for cutting genomic or plasmid DNA at a greater number of specific sites and in different settings.
5 cl, 11 dwg, 3 ex
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