FIELD: genetics.
SUBSTANCE: group of inventions refers to genetic engineering and can be used for expression of Cas9 nuclease in industrially valuable strains Debaryomyces hansenii. Disclosed is a genetic construct of pDhCas9sgRNA with SEQ ID NO.1 based on a CRISPR/Cas9 genome editing system which codes Cas9 nuclease specifically imported into cells Debaryomyces hansenii. Also, by transforming the yeast Debaryomyces hansenii Y-3014 with said plasmid, a recombinant yeast strain Debaryomyces hansenii pDhCas9sgRNA expressing Cas9 nuclease is obtained.
EFFECT: inventions provide efficient synthesis of Cas9 nuclease in yeast and efficient editing of chromosomal DNA Debaryomyces hansenii.
2 cl, 5 dwg, 3 ex
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