FIELD: biotechnology.
SUBSTANCE: invention relates to the development of a method for rapid quantitative determination of the level of humoral immunity of animals to strain O No. 2311/Zabaikalsky/2016 of the genotype O/ME-SA/Ind-2001 of the foot-and-mouth disease virus after vaccination using a liquid-phase "sandwich" ELISA variant. The proposed method involves the use of immunospecific and non-specific components, allows to determine the percentage of inhibition and quantify the level of humoral immunity of animals against strain O No. 2311/Zabaikalsky/2016 genotype O/ME-SA/Ind-2001 after vaccination. The developed method is characterized by high diagnostic sensitivity, specificity and accuracy for the quantitative determination of antibody titer against FMDV strain O No. 2311/Zabaikalsky/2016 genotype O/ME-SA/Ind-2001.
EFFECT: invention provides obtaining a result within 4 hours with high reliability.
2 cl, 4 dwg, 7 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| TEST SYSTEM BASED ON LIQUID PHASE BLOCKING INDIRECT "SANDWICH" ELISA FOR DETERMINATION OF ANTIBODY TITER AGAINST FMDV STRAIN O N2047/SAUDI ARABIA/2008 GENOTYPE O/ME-SA/PANASIA2 IN ANIMAL BLOOD SERA | 2022 |
|
RU2791614C1 |
| TEST SYSTEM FOR DETERMINING TITRE OF ANTIBODIES AGAINST COMPLETE VIRAL PARTICLES OF FMD VIRUS "O/KENYA/2017" STRAIN USING LIQUID-PHASE "SANDWICH" VERSION OF ELISA | 2023 |
|
RU2821894C1 |
| TEST SYSTEM FOR DETERMINING TITRE OF ANTIBODIES AGAINST STRUCTURAL PROTEINS OF FOOT-AND-MOUTH DISEASE VIRUS OF "A/TANZANIA/2013" STRAIN USING LIQUID-PHASE ENZYME IMMUNOASSAY | 2023 |
|
RU2818811C1 |
| TEST SYSTEM FOR QUANTITATIVE DETECTION OF ANTIBODIES AGAINST 146S COMPONENT OF FOOT-AND-MOUTH DISEASE VIRUS GENOTYPE O/EA-3 IN ANIMAL BLOOD SERUM BASED ON LIQUID-PHASE ENZYME IMMUNOASSAY | 2023 |
|
RU2817382C1 |
| TEST SYSTEM FOR DETECTING ANTIBODIES TO STRUCTURAL PROTEINS OF FOOT-AND-MOUTH DISEASE VIRUS STRAIN "SAT-1/KENYA/2017" USING LIQUID-PHASE BLOCKING INDIRECT "SANDWICH" VERSION OF ELISA | 2023 |
|
RU2821044C1 |
| TEST SYSTEM FOR DETECTING ANTIBODIES TO STRUCTURAL PROTEINS OF STRAIN "SAT-2/ERITREA/1998" OF FOOT-AND-MOUTH DISEASE VIRUS IN LIQUID-PHASE ENZYME IMMUNOASSAY | 2023 |
|
RU2825453C1 |
| TEST SYSTEM FOR DETERMINING TITER OF ANTIBODIES AGAINST 146S PARTICLES OF FOOT-AND-MOUTH DISEASE VIRUS GENOTYPE SAT-2/IV USING ELISA LIQUID-PHASE | 2023 |
|
RU2811996C1 |
| TEST SYSTEM BASED ON LIQUID-PHASE BLOCKING INDIRECT “SANDWICH” ELISA VARIANT FOR DETERMINING TITER OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS STRAIN “O-N2356/PAKISTAN/2018” OF GENOTYPE O/ME-SA/PANASIA2 ANT-10 IN ANIMAL BLOOD SERUM | 2023 |
|
RU2812210C1 |
| CULTURAL INACTIVATED EMULSION VACCINE AGAINST FOOT AND MOUTH DISEASE FROM O NO. 2620/ORENBURGSKY/2021 STRAIN OF O/ME-SA/IND-2001E GENOTYPE | 2023 |
|
RU2815537C1 |
| TEST SYSTEM FOR DETERMINING THE LEVEL OF ANTIBODIES AGAINST THE ANTIGEN OF FOOT-AND-MOUTH DISEASE VIRUS GENOTYPE A/ASIA/SEA-97 USING A LIQUID-PHASE BLOCKING INDIRECT “SANDWICH” VARIANT OF ELISA | 2023 |
|
RU2804845C1 |
