FIELD: biotechnology.
SUBSTANCE: invention relates to Rich, a new strain of the coronavirus enteritis virus of dogs of the Coronaviridae family of Alphacoronavirus genus deposited in the All-Russian State Collection of Exotic Types of Foot and Mouth Virus and Other Animal Pathogens (GKSHM) FSBI "ARRIAH" under registration number No. 376-dep/22-10. The strain presented is reproduced in transplantable feline kidney cell cultures (CRFK) and in continuous feline spleen cell culture (FS). In a continuous culture of CRFK cat kidney cells, within 72 hours of incubation, the titer of infectious activity of the virus reaches 3.42±0.14 lg TCD50/cm3, maintaining the original characteristics when passaged in cell culture for 5 passages.
EFFECT: strain presented can be used for the manufacture of biological products for the diagnosis and specific prevention of coronavirus enteritis in dogs and for the control of the antigenic activity of vaccines against this disease.
1 cl, 3 dwg, 5 tbl, 7 ex
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