METHOD OF OBTAINING EXOSOMES ORIGINING FROM MESENCHYMAL STEM CELLS AND A CULTURAL SOLUTION PRODUCED FROM THEM Russian patent published in 2023 - IPC C12N5/775 A61K35/28 A61K8/98 A61K8/14 A61P17/00 

Abstract RU 2799432 C1

FIELD: cell biology.

SUBSTANCE: invention relates to a method of obtaining exosomes derived from mesenchymal stem cells and a cell culture obtained from them, including the following steps: obtaining a cell sample from mesenchymal stem cells and its subculturing; culturing the subcultured cells in a substrate medium containing an enzyme that inhibits protein synthesis to obtain a cell culture; and separating the exosomes from the cell culture.

EFFECT: advantage of the method of producing exosomes of the invention is characterized by the fact that exosomes can be isolated in high concentration with high purity.

8 cl, 10 dwg, 4 tbl, 5 ex

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RU 2 799 432 C1

Authors

Kim, Jae Young

Chwae, Yong Joon

Dates

2023-07-05Published

2020-06-29Filed