FIELD: biotechnology.
SUBSTANCE: invention relates to a method of obtaining a potato plant with tetraallelic mutations in the coding region of EDR1 gene using CRISPR/Cas9 plant genome editing technology. The method involves obtaining a construct pKSE401-sgRNA2-2-1 containing the nucleotide sequence of an RNA conductor, transferring this construct into potato cells using Agrobacterium tumefaciens to introduce mutations in the coding region of the EDR1 gene, and selecting regenerative plants with tetraallelic mutations in the coding region of EDR1 gene.
EFFECT: invention is effective for obtaining a potato plant.
2 cl, 4 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2817384C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817374C2 |
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|
RU2817376C2 |
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|
RU2817379C2 |
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|
RU2817383C2 |
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RU2824558C2 |
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RU2814154C1 |
| METHOD FOR DETECTING GBSSI GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES | 2023 |
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| MOLECULE OF RNA-CONDUCTOR SGRNA FOR INTRODUCING MUTATIONS INTO CONSERVED REGION OF COMMON WHEAT PPD-D1 GENE PROMOTER REGION USING CRISPR/CAS9 GENOME EDITING SYSTEM | 2024 |
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RU2822358C1 |
