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2 824 558

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IPC

C12N15/05(2006-01-01)

C12N15/82(2006-01-01)

C12N15/11(2006-01-01)

C12N15/90(2006-01-01)

C12N9/22(2006-01-01)

A01H6/82(2018-01-01)

(21) (22)

Application

2022123793, 2022-09-07

(24)

Start date

2022-09-07

(22)

Actual filing date

2022-09-07

(45)

Published

2024-08-12

(72)

Inventor

Divashuk Mikhail GeorgievichZlobin Nikolaj EvgenevichIvanova Lyubov AleksandrovnaKarlov Gennadij IlichKomakhin Roman AleksandrovichKonovalova Lyudmila NikolaevnaLebedeva Marina ValerevnaSamarina Mariya AlekseevnaTaranov Vasilij Vasilevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Nauchno-Issledovatelskij Institut Selskokhozyajstvennoj Biotekhnologii" Vniisb)

METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/Cas9 PLANT GENOME EDITING METHOD Russian patent published in 2024 - IPC C12N15/05 C12N15/82 C12N15/11 C12N15/90 C12N9/22 A01H6/82

Abstract RU 2824558 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of obtaining a potato plant with biallelic mutations in the coding region of EDR1 gene using CRISPR/Cas9 plant genome editing technology. The method involves obtaining a construct pKSE401-EDR1-1-2-1 containing the nucleotide sequence of an RNA conductor, transferring this construct into potato cells using Agrobacterium tumefaciens to introduce mutations in the coding region of the EDR1 gene, and selecting regenerative plants with biallelic mutations in the coding region of EDR1 gene.

EFFECT: invention is effective for producing potato plants using the above method.

2 cl, 3 dwg, 1 ex

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RU 2 824 558 C2

Authors

Divashuk Mikhail Georgievich

Zlobin Nikolaj Evgenevich

Ivanova Lyubov Aleksandrovna

Karlov Gennadij Ilich

Komakhin Roman Aleksandrovich

Konovalova Lyudmila Nikolaevna

Lebedeva Marina Valerevna

Samarina Mariya Alekseevna

Taranov Vasilij Vasilevich

Dates

2024-08-12—Published

2022-09-07—Filed