FIELD: biotechnology.
SUBSTANCE: invention relates to a method of obtaining a potato plant with tetraallelic mutations in the coding region of EDR1 gene using CRISPR/Cas9 plant genome editing technology.
EFFECT: method involves obtaining a construct pKSE401-sgRNA2-2-1 containing the nucleotide sequence of an RNA conductor, transferring this construct into potato cells using Agrobacterium tumefaciens to introduce mutations in the coding region of the EDR1 gene, and selecting regenerative plants with tetraallelic mutations in the coding region of EDR1 gene.
2 cl, 3 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/Cas9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817378C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2814154C1 |
| METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2817384C2 |
| METHOD OF OBTAINING A POTATO PLANT WITH TETRAALLEL MUTATIONS IN THE EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2800828C1 |
| METHOD FOR OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817379C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817374C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817376C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/Cas9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2824558C2 |
| MOLECULE OF RNA-CONDUCTOR SGRNA FOR INTRODUCING MUTATIONS INTO CONSERVED REGION OF COMMON WHEAT PPD-D1 GENE PROMOTER REGION USING CRISPR/CAS9 GENOME EDITING SYSTEM | 2024 |
|
RU2822358C1 |
| METHOD FOR PRODUCING A WHEAT PLANT WITH BIALLEL MUTATIONS IN THE PROMOTOR REGION OF THE VRN-A1 GENE USING THE CRISPR/CAS9 TECHNOLOGY FOR EDITING PLANT GENOME | 2020 |
|
RU2772575C2 |
