METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY Russian patent published in 2024 - IPC C12N15/82 C12N15/63 C12N15/11 A01H5/00 

Abstract RU 2817383 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of obtaining a potato plant with tetraallelic mutations in the coding region of EDR1 gene using CRISPR/Cas9 plant genome editing technology.

EFFECT: method involves obtaining a construct pKSE401-sgRNA2-2-1 containing the nucleotide sequence of an RNA conductor, transferring this construct into potato cells using Agrobacterium tumefaciens to introduce mutations in the coding region of the EDR1 gene, and selecting regenerative plants with tetraallelic mutations in the coding region of EDR1 gene.

2 cl, 3 dwg

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RU 2 817 383 C2

Authors

Blinkov Andrej Olegovich

Divashuk Mikhail Georgievich

Zlobin Nikolaj Evgenevich

Ivanova Lyubov Aleksandrovna

Karlov Gennadij Ilich

Komakhin Roman Aleksandrovich

Konovalova Lyudmila Nikolaevna

Lebedeva Marina Valerevna

Taranov Vasilij Vasilevich

Dates

2024-04-15Published

2022-09-07Filed