FIELD: biotechnology.
SUBSTANCE: invention relates to a method of obtaining a potato plant with tetraallelic mutations in the coding region of EDR1 gene using CRISPR/Cas9 plant genome editing technology.
EFFECT: invention makes it possible to obtain a potato plant, and the method includes the following steps: obtaining pKSE401-sgRNA2-2-1 construct containing the nucleotide sequence of the guide RNA, transferring this construct into potato cells using Agrobacterium tumefaciens bacterium to introduce mutations in the coding region of EDR1 gene and selection of regenerated plants with a nucleotide deletion in the coding region of EDR1 gene.
2 cl, 4 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2817384C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817374C2 |
| METHOD OF OBTAINING A POTATO PLANT WITH TETRAALLEL MUTATIONS IN THE EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2800828C1 |
| METHOD FOR OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817379C2 |
| METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
RU2817383C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2814154C1 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/Cas9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2824558C2 |
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/Cas9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817378C2 |
| METHOD FOR PRODUCING A WHEAT PLANT WITH BIALLEL MUTATIONS IN THE PROMOTOR REGION OF THE VRN-A1 GENE USING THE CRISPR/CAS9 TECHNOLOGY FOR EDITING PLANT GENOME | 2020 |
|
RU2772575C2 |
| METHOD FOR OBTAINING WHEAT PLANT WITH NUCLEOTIDE DELETION IN PROMOTOR REGION OF VRN-A1 GENE USING CRISPR/CAS9 TECHNOLOGY OF PLANT GENOME EDITING | 2020 |
|
RU2772577C2 |
