FIELD: genetic engineering and biotechnology.
SUBSTANCE: method of obtaining a potato plant with biallelic mutations in EDR1 gene encoding region using CRISPr/Cas9 plant genome editing method. Obtaining a construct based on the pKSE401 vector containing the nucleotide sequence of the guide RNA SEQ ID NO: 3. The construct is transferred into potato cells using the Agrobacterium tumefaciens bacterium. Regenerated plants with triallelic mutations in the coding region of the EDR1 gene are selected.
EFFECT: invention makes it possible to obtain potato plants with triallelic mutations in the coding region of the EDR1 gene for further use of the plant in the breeding process.
3 cl, 4 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING POTATO PLANT WITH BIALLELIC MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING METHOD | 2022 |
|
RU2817374C2 |
| METHOD OF OBTAINING POTATO PLANT WITH TETRAALLEL MUTATIONS IN EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
|
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RU2817384C2 |
| METHOD OF OBTAINING A POTATO PLANT WITH TETRAALLEL MUTATIONS IN THE EDR1 GENE ENCODING REGION USING CRISPR/CAS9 PLANT GENOME EDITING TECHNOLOGY | 2022 |
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