FIELD: biotechnology.
SUBSTANCE: vector is provided for transformation into an expressing host cell containing a polynucleotide sequence comprising a nucleotide sequence that is at least 97% identical to the nucleotide sequence of SEQ ID NO: 4 or 5, wherein the nucleotide sequence encodes a functional glucoamylase enzyme. Also a recombinant host cell for expression of a polypeptide of interest, containing the above vector is proposed.
EFFECT: invention provides obtaining glucoamylases with increased stability and activity, as well as improved tolerance to low pH values.
6 cl, 1 dwg, 13 tbl, 11 ex
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