FIELD: biotechnology.
SUBSTANCE: invention relates to recombinant pNhyDAAO vector for expression of D-amino acid oxidase of Natronosporangium hydrolyticum ACPA39 bacteria, and to recombinant Escherichia coli BL21(DE3)/pNhyDAAO strain containing this recombinant vector.
EFFECT: invention is effective for use in fine organic synthesis, selective determination of D-Leu, D-Met and D-Phe, etc. in complex biological systems, including human fluids and tissues.
2 cl, 4 dwg, 6 tbl, 4 ex
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