FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to genetic engineering, and represents recombinant plasmid DNA pNhaDAAO containing a modified Nhadaao gene coding D-amino acid oxidase Natrarchaeobius halalkaliphilus archaea AArcht4 (NhaDAAO). Obtained plasmid provides expression of the modified gene included in its nucleotide sequence in Escherichia coli cells. As a result of transformation of the strain E. coli BL21 (DE3) with the proposed plasmid, a new recombinant producer strain E. coli BL21 (DE3)/pNhaDAAO VKPM B-14556 is obtained, which provides expression of NhaDAAO in an active form.
EFFECT: invention enables to obtain NhaDAAO in an active form with high efficiency.
2 cl, 4 dwg, 5 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMIDE pNhyDAAO PROVIDING SYNTHESIS OF D-AMINO ACID OXIDASE OF NATRONOSPORANGIUM HYDROLYTICUM ACPA39 (NhyDAAO) BACTERIA IN ESCHERICHIA COLI CELLS AND RECOMBINANT STRAIN ESCHERICHIA COLI BL21(DE3)/pNhyDAAO VKPM V-143 37 - NhyDAAO PRODUCER | 2022 |
|
RU2803295C1 |
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|
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|
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| RECOMBINANT STRAIN Escherichia coli-PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS FROM Xanthomonas rubrilineans, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2499830C1 |
| MUTANT OXIDASE OF D-AMINO ACIDS | 2009 |
|
RU2412998C1 |
| MUTANT D-AMINOACID OXIDASES | 2007 |
|
RU2362806C2 |
| MUTANT OXIDASE OF D-AMINO ACIDS (VERSIONS) | 2012 |
|
RU2507262C1 |
| STRAIN Escherichia coli - PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS FROM Xanthomonas rubrilineans, AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2502797C1 |
| RECOMBINANT DNA ENCODING FUNCTIONALLY ACTIVE HYBRID PROTEIN OF D-AMINO ACID OXYDASE WITH CHITIN-COUPLING DOMAIN (DAOcbd), pVR1 RECOMBINANT PLASMID PROVIDING SYNTHESIS THEREOF IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C 41 (DE3)/pVR1 AS PRODUCER OF DAOcbd | 2006 |
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| RECOMBINANT DNA, WHICH CODES FUNCTIONALLY ACTIVE HYBRID PROTEIN GL7ACA-ACYLASE WITH CHITIN-BINDING DOMAIN (BrdGL7ACA-cbd) AND RECOMBINANT PLASMID pSVH0108, PROVIDING FOR ITS SYNTHESIS IN CELLS Escherichia coli, RECOMBINANT STRAIN Escherichia coli BL21(DE3)/pSVH0108-PRODUCER BrdGL7ACA-cbd | 2006 |
|
RU2388826C2 |
