METHOD OF QUANTITATIVE DETERMINATION OF ANTIHYPERTENSIVE AGENTS IN BLOOD PLASMA Russian patent published in 2023 - IPC G01N30/14 G01N30/24 G01N30/34 G01N30/72 G01N1/28 

FIELD: analytical chemistry.

SUBSTANCE: method of the simultaneous quantitative determination of lisinopril, indapamide, valsartan, amlodipine, and metoprolol in blood plasma including the use of the HPLC-MS/MS method, is disclosed. Sample preparation is carried out with the precipitation of proteins by mixing 200 μl of a blood plasma sample with 600 μl of acetonitrile, followed by shaking for 10 minutes and further centrifugation at 19,000g for 10 minutes at a temperature of + 4°C, followed by injection of 5 μl of the sample using an autosampler and separation on a UCT Selectra C18 4.6 mm column×100 mm, 3um, 100A with precolumn Selectra C18 Guard Cartridges SLC-18GDC46-3UM at a temperature of 35°C, in a gradient elution mode in the ratio of 0.1% formic acid solution/acetonitrile: 0 min — 80%/20%, 0.1 min — 45%/55%, 5 min — 10%/90%, 10 min — 80%/20%, with a flow rate of 300 µl/min and detection in positive mode electrospray ionization, electrospray voltage 4,000 V, shell gas 50 arb, auxiliary gas 10 arb, purge gas 1 arb, evaporator temperature 350°C, ion transport tube temperature — 300°C, using MRM mode with argon supply pressure of 2 mTorr.

EFFECT: invention provides a sensitive, selective, accurate and precision method for the quantitative determination of the specified mixture of substances in blood plasma.

6 cl, 1 tbl