FIELD: biotechnology.
SUBSTANCE: set of oligonucleotide primers is presented for determining methylation of the human CXCR4 gene promoter in samples of biological material. The kit includes direct primer SEQ ID NO: 1 E-CXCR4-F1 - GTG GTT ATT GGA GTA TTT AGG T, reverse primer SEQ ID NO: 2 E-CXCR4-R1 - TCT ACC CCT CTC CCC CA, sequencing primer SEQ ID NO: 3 E-CXCR4-S - GTT AAT AAA TTG AAG TTT TTG GT.
EFFECT: synthesized oligonucleotides SEQ ID NO NO: 1-3 do not cross-react with other genome sequences and amplify a sequence including target methylated loci in the CCR5 gene promoter with 100% specificity.
5 cl, 1 tbl, 3 ex
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