FIELD: biotechnology.
SUBSTANCE: set of oligonucleotide primers is presented for determining methylation of the human CCR5 gene promoter in samples of biological material. The set includes forward primer 2CCR5-Fp - TAT GAT TGA TTT GTA TAG TTT ATT TGG TTA, reverse primer 2CCR5-Rp - CTC ATC TCA AAA ACT AAC TAA C, sequencing primer 2CCR5-S - GGT TAG AAG AGT TGA GAT ATT.
EFFECT: synthesized oligonucleotides do not cross-react with other genome sequences and amplify the sequence including target methylated loci in the CCR5 gene promoter with 100% specificity.
5 cl, 1 tbl, 3 ex
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