NANOAGENTS FOR SCREENING PATHOGENIC STRAINS OF MULTIDRUG-RESISTANT BACTERIA AND METHOD OF PREPARATION Russian patent published in 2023 - IPC C12Q1/00 C12Q1/70 C12N9/10 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and is a nanoagent for screening pathogenic strains of multidrug-resistant bacteria, characterized in that it is a conjugate of gold nanoparticles with the enzyme bacteriophage or profage depolymerase immobilized on the surface of the nanoparticle, characterized in that the sizes of gold nanoparticles range from 20 nm to 32+4 nm, the enzyme is a depolymerase Dpo1053 encoded in the genome of the lytic phage Acinetobacter baumannii AP22, or the enzyme is depolymerase Dpo1432 encoded in the genome of the lytic phage Acinetobacter baumannii vB_AbaP_AS12. The invention also concerns a method for obtaining such a nanoagent for screening pathogenic strains of multidrug-resistant bacteria, including the stages of obtaining gold nanoparticles, obtaining conjugates of gold nanoparticles with a phage or profage enzyme depolymerase, characterized by immobilization of enzymes on the surface of gold nanoparticles, incubation of gold nanoparticle conjugates with an enzyme during ultrasound treatment, addition of bovine serum albumin and washing of the obtained nanoagents at least three times, and the sizes of gold nanoparticles range from 20 nm to 32+4 nm, the enzyme is depolymerase Dpo1053 encoded in the genome of the lytic phage Acinetobacter baumannii AP22, or the enzyme is depolymerase Dpo1432 encoded in the genome of the lytic phage Acinetobacter baumannii vB_AbaP_AS12.

EFFECT: invention provides the preparation of a nanoagent for the accelerated determination of a strain of pathogenic bacteria.

16 cl, 5 dwg