METHOD OF OBTAINING DRY ERYTHROCYTE ANTHRAX ANTIGEN DIAGNOSTICUM Russian patent published in 2023 - IPC G01N33/531 G01N33/556 A61K39/07 

FIELD: biotechnology; medical microbiology.

SUBSTANCE: following has been proposed: a method of obtaining an erythrocyte anthrax antigen diagnosticum including activation of formalinized sheep erythrocytes with a conjugating drug consisting of sodium alkyl sulfate and a freshly prepared solution of tannin in phosphate buffer solution (PBS) pH 7.2 in final concentrations of 2.5% (1:40) and 0 .0025% (1:40,000) respectively; then the red blood cells are incubated for 20 minutes in a water bath at 45°C, washed with PBS pH 7.2, suspended in Zerensen phosphate-buffered saline pH 5.9, a concentrated fraction of the cultural filtrate of the vaccine strain Bacillus anthracis 55 with a molecular weight of more than 10 kDa and a protein content of 0.5 to 0.125 mg/ml are added and incubated for 40 min at 45°C, formalin is added to a final concentration of 1%, then placed in the refrigerator at 4°C for 18 hours, then washed three times by centrifugation at 1,500 rpm for 10 minutes in PBS pH 7.2 containing 1% formaldehyde, washed erythrocytes are suspended in a drying medium to a 5% concentration and lyophilized in accordance with the proposed regime.

EFFECT: invention provides an expansion of the arsenal of methods for obtaining erythrocyte anthrax antigen diagnosticum for quantitative determination of the level of anthrax antibodies in the sera of humans and animals.

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