FIELD: biotechnology.
SUBSTANCE: invention relates to a method for isolating microspores to obtain doubled haploids of the Brassicaceae family in an in vitro culture of microspores, which consists in individual preparation of the buds with a scalpel cross-section, subsequent immersion of the halves of the buds in sterile test tubes with a nutrient medium and shaking in a rotary shaker for 10 -60 seconds.
EFFECT: invention allows minimizing the mechanical impact on the bud tissues.
1 cl, 4 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING DIHAPLOID BARLEY PLANTS FROM CULTURED MYCOSPORS IN VITRO | 2013 |
|
RU2557389C2 |
| METHOD FOR OBTAINING REGENERANT PLANTS BRASSICA OLERACEA L. IN VITRO | 2021 |
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RU2759735C1 |
| METHOD OF DOUBLING THE CHROMOSOME SET OF RAPESEED HAPLOIDS (BRASSICA NAPUS L.) | 2022 |
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RU2808269C1 |
| METHOD OF PRODUCING BRASSICA REGENERATION PLANTS IN VITRO | 2020 |
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RU2741647C1 |
| METHOD FOR OBTAINING DOUBLED HAPLOIDS OF CARROTS IN CULTURE OF ISOLATED MICROSPORES IN VITRO | 2020 |
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RU2750959C1 |
| METHOD FOR CREATING DOUBLED HAPLOIDS OF WHITE CABBAGE (BRASSICA OLERACEA L.) IN A CULTURE OF ISOLATED MICROSPORES | 2021 |
|
RU2769815C1 |
| METHOD OF PRODUCING HAPLOID PLANTS-REGENERANTS OF REPRODUCTIVE ORGANS OF BRASSICA OLERACEA LINNAEUS IN VITRO | 2015 |
|
RU2607007C1 |
| METHOD FOR PREPARING COLZA (BRASSICA NAPUS L) FERTILITY RESTORER | 2007 |
|
RU2366705C2 |
| METHOD FOR CULTIVATION OF ISOLATED ANTHERS OF SPRING RAPE PLANTS | 2006 |
|
RU2314680C2 |
| METHOD FOR MULTI-STAGE SURFACE STERILIZATION OF UPPER PARTS OF GENERATIVE SHOOTS OF BETA VULGARIS L. BEFORE INTRODUCTION INTO IN VITRO CULTURE | 2022 |
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RU2796463C1 |
