FIELD: agriculture.
SUBSTANCE: invention discloses a kit and a new method of extracting progesterone metabolite from a fecal/manure sample. The invention also discloses a paper-based microfluidic device for detecting progesterone metabolite for early pregnancy detection in cattle and buffaloes. The invention also discloses antibodies developed against the said metabolite, an extraction method, methods of making paper-based microfluidic devices and methods of inexpensive, rapid and non-invasive detection of early pregnancy in cattle and buffaloes.
EFFECT: obtaining non-invasive paper-based device for determining pregnancy in cattle and buffalo.
17 cl, 10 dwg, 2 tbl
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