FIELD: medicine, molecular biology, diagnosis.
SUBSTANCE: claimed method includes virion destroying by heating in lytic buffer, lysat fractioning by centrifugation in presence of sodium perchlorate, RNA deposition with cooled isopropyl alcohol. Method of present invention is useful in diagnosis of intestinal viral infections and investigation of detected by RT-PCR method genome sequences.
EFFECT: method of increased sensitivity.
1 ex, 3 dwg, 2 tbl
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Authors
Dates
2007-12-27—Published
2006-04-10—Filed