METHOD FOR ISOLATION OF INTESTINAL VIRUS RNA FOR ANALYSIS BY RT-PCR METHOD Russian patent published in 2007 - IPC G01N33/50 C12Q1/68 

Abstract RU 2313792 C1

FIELD: medicine, molecular biology, diagnosis.

SUBSTANCE: claimed method includes virion destroying by heating in lytic buffer, lysat fractioning by centrifugation in presence of sodium perchlorate, RNA deposition with cooled isopropyl alcohol. Method of present invention is useful in diagnosis of intestinal viral infections and investigation of detected by RT-PCR method genome sequences.

EFFECT: method of increased sensitivity.

1 ex, 3 dwg, 2 tbl

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Authors

Novikova Nadezhda Alekseevna

Golitsyna Ljudmila Nikolaevna

Epifanova Natalija Vladimirovna

Fedorova Oksana Fedorovna

Lukovnikova Ljubov' Borisovna

Dates

2007-12-27Published

2006-04-10Filed