FIELD: biotechnology.
SUBSTANCE: method for the enzymatic production of sulfur-containing amino acids is proposed, comprising the steps of: a) microorganism of Enterobacteriaceae family or microorganism of Corynebacteriaceae family is obtained in which the gene encoding the polypeptide with thiosulfate-sulfotransferase activity is overexpressed; b) fermenting the microorganism obtained in step a) in a medium that contains an inorganic sulfur source selected from the group consisting of a thiosulfuric acid salt or a mixture of a thiosulfuric acid salt and a sulfuric acid salt to obtain a fermentation broth; and c) concentrating the sulfur-containing amino acid in the fermentation broth obtained in step b). Microorganism Corynebacterium glutamicum, which secretes or produces L-methionine and overexpresses the gene encoding a polypeptide with thiosulfate-sulfotransferase activity, is proposed. Microorganism Escherichia coli, which secretes or produces L-methionine and overexpresses the gene encoding a polypeptide with thiosulfate-sulfotransferase activity, is proposed.
EFFECT: group of inventions allows to increase production of sulfur-containing amino acids, in particular L-methionine.
16 cl, 1 dwg, 4 tbl, 7 ex
