METHOD OF PREPARING AND PRESERVING CLEARED ANATOMICAL PREPARATIONS Russian patent published in 2024 - IPC A01N1/02 G09B23/30 

FIELD: medicine; topographic anatomy.

SUBSTANCE: method of preparing and preserving cleared anatomical preparations is proposed, the said method includes dissecting the anatomical material and fixing it in 10 vol.% solution of neutral formaldehyde, according to the invention, the material is dehydrated and degreased using technical acetone, objects up to 10 mm thick are kept in acetone for three days, while the acetone is replaced every day, then the material is clarified with petroleum xylene for three days with a daily change of xylene, after which is left in the fourth portion of xylene for 30 days, and objects more than 10 mm thick, entire organs, organ complexes are kept for six days in three portions of acetone, while the acetone is replaced once every two days, then the material is clarified with petroleum xylene, keeping the material for six days in three portions of xylene, while the xylene is replaced once every two days, after which it is left in the fourth portion of xylene for 60 days, then the material is removed and dried in a thermostat at 60°C for an hour, and then in air for 30 minutes, then the preparation is placed in a silicone mould, the dimensions of which correspond to the size of the prepared anatomical specimen, and it is enclosed in pre-prepared jewellery epoxy resin so that the enclosed object is located in the thickness of the epoxy layer, after In order to achieve complete curing of the epoxy resin, the filled silicone mould is placed in a thermostat for three days at a temperature of 40°C.

EFFECT: use of the invention makes it possible to develop a method and preserve cleared anatomical preparations of various sizes while maintaining the features of their topographic-anatomical characteristics and original size.

1 cl, 7 dwg, 1 ex