BONE PLASTIC MATERIAL WITH CONTROLLED PROPERTIES, METHOD OF ITS PRODUCTION AND USE Russian patent published in 2024 - IPC A61L27/24 A61F2/28 A61K35/32 A61P19/00 

FIELD: medicine.

SUBSTANCE: group of inventions relates to a bone plastic material (BPM) for stimulating reparative processes in bone tissue defects, which is a material based on collagen and bone chips, characterized by it containing human type 1 collagen, isolated from the tendons of tissue donors, in an amount of 10–16 wt.%, bone chips obtained from tissue donors in an amount of 83–89 wt.%, while the composition uses a 0.8% solution of collagen and bone chips with a particle size of 700–800 microns, or a 1.1% solution collagen and bone chips with a particle size of 80–800 microns; collagen and collagen fibres in the composition of BPM are characterized by an autofluorescence level of not more than 40 foot-candles (FC), also refers to a method of producing bone plastic material (BPM), including mixing collagen and bone chips with subsequent lyophilisation and sterilization of the resulting mixture, characterized by the following: for mixing 0.8% solution of human type 1 collagen isolated from the tendons of tissue donors by acid extraction is used, and bone chips with a particle size of 700–800 microns, or a 1.1% solution of human type 1 collagen isolated from the tendons of tissue donors by acid extraction, and bone chips with a diameter of 80–180 microns, while 5.5 to 6.5 grams of bone chips are taken per 100 ml of collagen, after lyophilisation, the quality of the resulting BPM is checked using fluorescent microscopy, when an autofluorescence level of 40 is obtained FC and less obtained BPM are considered suitable for subsequent use to stimulate reparative processes in bone tissue defects, also applies to the use of BPM to stimulate reparative processes in bone tissue defects, including preliminary soaking of BPM in an isotonic solution of sodium chloride (0.9% NaCl) in for at least 30 minutes followed by filling the bone tissue defect.

EFFECT: ensuring the development of a low-adhesive BPM graft for human cells based on tissue donor material, characterized by a cell density on the BPM surface of not more than 2 thousand per cm², the absence of spread cells and cells with a wide lamella (when cell lines are cultured for 3 days in the presence of BPM), while ensuring the homogeneity of the components of the mixture, characterized by the absence of crumbs and collagen in the entire volume of the mixture.

5 cl, 6 dwg, 5 tbl