BONE-PLASTIC MATERIAL WITH CONTROLLED PROPERTIES, A METHOD OF ITS PRODUCTION AND USE Russian patent published in 2024 - IPC A61L27/24 A61F2/28 A61K35/32 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of producing bone plastic material (BPM) to stimulate reparative processes in bone tissue defects, which is a sponge of a flattened, cylindrical or cubic shape, including mixing collagen and bone chips with subsequent lyophilization and sterilization of the resulting mixture, characterized in that For mixing, a solution of human type 1 collagen, isolated from the tendons of tissue donors by acid extraction, and bone chips obtained from tissue donors are used, where from 5.5 to 6.5 grams of bone chips are taken per 100 ml of collagen, and to obtain a highly adhesive for human cells of material with a high homogeneity of distribution of bone chips, a 0.8% collagen solution and bone chips of 500–630 microns in size are used; to obtain a highly adhesive material for human cells with an average homogeneity of distribution of bone chips, a 0.8% collagen solution and bone chips of size 250–315 microns, to obtain a highly adhesive material for human cells with low homogeneity of distribution of bone chips, use a 0.8% solution of collagen and bone chips 80–180 microns in size, to obtain a low-adhesive material with a high homogeneity of distribution of bone chips, use a 0.8% solution collagen and bone chips measuring 700–800 microns or 1.1% collagen solution and bone chips measuring 80–180 microns; to obtain a low-adhesive material with low homogeneity of distribution of bone chips, use a 1.1% collagen solution and bone chips measuring 250–800 microns.

EFFECT: invention provides the production of a BPM graft based on the material of tissue donors, with the possibility of changing the biological properties at the stage of BPM production by changing the composition of the BPM, namely the parameters of the components used — the size of bone chips and the percentage of type 1 collagen, with the production of BPM that is highly adhesive for human cells, characterized by a cell density on the surface of the BPM of 4 thousand per cm² and higher, the presence of spread out cells and cells with a wide lamella, or a low-adhesive BPM for human cells, characterized by a cell density on the surface of the BPM of no more than 2 thousand per cm², the absence of spread cells and cells with a wide lamella, with a given homogeneity of the distribution of bone chips (high, medium or low).

4 cl, 6 dwg, 4 tbl