METHOD AND KIT FOR IDENTIFICATION OF VACCINIUM MYRTILLUS Russian patent published in 2024 - IPC C12N15/82 

FIELD: biotechnology.

SUBSTANCE: method of identifying Vaccinium myrtillus in a botanical mixture by PCR amplification is described. A V. myrtillus nucleic acid fragment located within internal transcribed spacer 1, the 5.8S ribosomal RNA gene region and internal transcribed spacer 2 is determined in the mixture sample. Nucleic acids are isolated from the specified sample. Real-time PCR is performed on the isolated nucleic acid using a set of primers selected from the following group: SEQ ID NO:5 and SEQ ID NO:6; SEQ ID NO:7 and SEQ ID NO:8; SEQ ID NO:9 and SEQ ID NO:10; SEQ ID NO:11 and SEQ ID NO:12. The probe is annealed to the region of the nucleic acid amplified by the primers, wherein the said probe consists of the sequence SEQ ID NO:13. The presence of an amplification product is determined. Detection of an amplification product indicates the presence of Vaccinium myrtillus in the botanical composition.

EFFECT: invention expands the range of agents for identifying Vaccinium myrtillus.

7 cl, 12 dwg, 10 tbl, 4 ex