FIELD: molecular biology.
SUBSTANCE: described is a set of synthetic oligonucleotide probes for amplifying the complete nucleotide sequence of sarcomeric proteins MYH7, TPM1, TNNT2 and TNNI3 by PCR of long DNA fragments. Kit is characterized by the fact that when it is used there is a targeted enrichment of the complete nucleotide sequence of the analysed genes, which serves as a basis for analysing variants throughout the sequence of genes, including introns and untranslated regions.
EFFECT: obtaining PCR products with length from 4665 to 8749 base pairs, overlapping with each other within one gene when aligned on the sequence of the human genome, intended for further sequencing and analysis of variability of genes of sarcomeric proteins.
1 cl, 2 dwg
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