MODIFIED Α1-Α2 DOMAINS OF NON-NATURAL NKG2D LIGANDS THAT BIND TO NON-NATURAL NKG2D RECEPTORS Russian patent published in 2024 - IPC C07K14/74 C07K14/705 C07K16/28 

FIELD: biotechnology.

SUBSTANCE: invention relates to a non-natural modified domain α 1-α 2 molecules of natural ligand NKG2D. Specified modified domainα 1-α 2 binds the non-natural NKG2D receptor and contains an amino acid sequence having at least 90% identity with the following SEQ ID NO: 4, wherein in the said amino acid sequence having at least 90% identity with the following SEQ ID NO: 4, positions corresponding to positions 8, 80, 154, 155, 156, 157, 158 and 159 of the following SEQ ID NO: 4, superseded from the following SEQ ID NO: 4). The invention provides high binding activity to the non-natural ectodomain of the NKG2D receptor containing the following amino acid sequence SEQ ID NO: 17 with substitutions Y75A and Y122F compared with (a) a non-natural NKG2D receptor ectodomain containing the following amino acid sequence SEQ ID NO: 17 having Y75A, and (b) the corresponding natural NKG2D receptor ectodomain containing the amino acid sequence SEQ ID NO: 17.

EFFECT: obtaining modified α1-α2 domains of non-natural nkg2d ligands that bind to non-natural nkg2d receptors.

11 cl, 18 dwg, 6 ex