FIELD: biotechnology.
SUBSTANCE: following is proposed: Komagataella phaffii yeast transformant producing an active form of Vicugna pacos alpaca chymosin and containing in chromosomal DNA an optimized synthetic Vicugna pacos alpaca prochymosin gene, the nucleotide sequence of which is given in the sequence listing under the following SEQ ID NO: 1. Also a strain of K. phaffii N416 RNCIM Y-5079 — a producer of Vicugna pacos alpaca chymosin in the active form is proposed.
EFFECT: expansion of the arsenal of yeast recombinant microorganisms that produce chymosin in active form.
2 cl, 7 dwg, 4 tbl, 4 ex
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|---|---|---|---|
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|
RU2815882C1 |
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