FIELD: biotechnology.
SUBSTANCE: disclosed is a transformant of yeast Komagataella phaffii, producing chymosin Vicugna pacos in an active form, containing in chromosomal DNA an optimized synthetic alpaca prochymosin gene Vicugna pacos, the nucleotide sequence of which is given in the sequence list under the number SEQ ID NO: 1, and the ScHAC1 gene from Saccharomyces cerevisiae. Also disclosed is a strain of Komagataella phaffii VKPM Y-5150, which is a producer of recombinant alpaca chymosin Vicugna pacos in an active form.
EFFECT: invention provides wider range of yeast recombinant microorganisms producing alpaca chymosin Vicugna pacos in active form.
2 cl, 2 dwg, 5 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
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