FIELD: medicine; surgery.
SUBSTANCE: invention can be used to assess recovery of excretory function of liver after antegrade decompression of bile passages in obstructive jaundice. Bile is used as a biological medium for analysis. Samples are examined four times: intraoperatively from a puncture needle and three times in the early postoperative period on 3rd, 7th, 10th day from a drainage catheter. Raman spectra are analyzed in the range of wave numbers from 1,000 to 1,800 cm−1 and recorded in to each sample, Raman spectra, corresponding to bilirubin in bands 1,255–1,260 and 1,606–1,620 cm−1. When determining amplitude of signal intensity in analyzed sample in bands 1,255–1,260 and 1,606–1,620 cm−1, equal to level of reference values 1,000–2,000 relative units, characterizing bilirubin peaks in patients without obstructive jaundice, recovery of excretory function of liver is determined.
EFFECT: method enables dynamic assessment of the functional state of the liver in the patients with obstructive jaundice after the primary stage of the surgical management by conducting a study of bile by Raman spectroscopy.
1 cl, 3 ex
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Authors
Dates
2024-03-26—Published
2023-05-24—Filed