FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to an expression vector pBMS10 based on LuxR-LuxI elements of a quorum sensing system (QS) of marine psychrophilic bacteria of the species Aliivibrio logei for cloning and expression of target genes in Escherichia coli cells. Invention enables to perform inducible expression realized by two methods: auto-induction at culturing temperature of 22 °C and induction by exogenous addition of 3OxoC6-HSL.
EFFECT: invention is effective for biosynthesis of recombinant proteins in cells of Escherichia coli with possibility of obtaining amounts of target protein making up to 50% of total protein in cell, and cultivation in temperature range 22–30 °C.
1 cl, 2 dwg, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF CONTROLLED EXPRESSION OF RECOMBINANT GENES IN ESCHERICHIA COLI CELLS USING LUXR-LUXI "QUORUM SENSING" ELEMENTS OF SYSTEM OF PSYCHROPHILIC BACTERIA OF ALIIVIBRIO GENUS | 2022 |
|
RU2810597C2 |
| BACTERIAL LUX-BIOSENSOR WITH INCREASED SENSITIVITY FOR DETECTION OF ACYL HOMOSERINE LACTONE DERIVATIVES | 2020 |
|
RU2777196C2 |
| GLOBAL REGULATORS OF BACTERIAL PATHOGENIC GENES, INACTIVATING PROTEIN OF BACTERIAL AUTOINDUCTORS SHOWING TARGETS FOR CREATURE FOR RESISTANCE TO DISEASES | 1999 |
|
RU2236462C2 |
| METHOD OF COUMARIN APPLICATION FOR INHIBITION OF DIFFERENT "QUORUM SENSING" LUX/LUXR TYPE SYSTEMS IN BACTERIA | 2019 |
|
RU2744456C1 |
| BACTERIAL COLLECTIVE BEHAVIOUR (QUORUM SENSING) REGULATOR | 2012 |
|
RU2534617C2 |
| METHOD OF USING GAMMA-OCTALACTONE AS AN INHIBITOR OF "QUORUM OF SENSING" SYSTEM LUXI/LUXR TYPE IN BACTERIA | 2017 |
|
RU2691634C2 |
| APPLICATION OF COUMARIN AND ITS DERIVATIVES AS INHIBITORS OF LuxI / LuxR TYPE "QUORUM SENSING" SYSTEM IN BACTERIA | 2015 |
|
RU2616237C1 |
| USE OF 1,3-BENZODIOXOLE DERIVATIVES AS BACTERIAL COLLECTIVE BEHAVIOUR (QUORUM SENSING) REGULATORS | 2012 |
|
RU2514001C2 |
| BACTERIOPHAGE N15 REPLICON-BASE VECTOR AND RECOMBINANT VECTOR FOR REGULATED EXPRESSION OF TARGET GENE IN ESCHERICHIA COLI CELLS, STRAIN ESCHERICHIA COLI PROVIDING POSSIBILITY FOR REGULATION OF VECTOR COPY NUMBER AND EXPRESSION SYSTEM | 2006 |
|
RU2312146C1 |
| SET OF STRESS-INDUCIBLE LUX BIOSENSORS BASED ON BACILLUS SUBTILIS CELLS FOR STUDYING TOXICITY MECHANISMS | 2022 |
|
RU2811895C2 |
