FIELD: biotechnology.
SUBSTANCE: described is a method for detecting aminoglycoside resistance genes from the aadA group in bacteria of animal origin by real-time PCR, involving polymerase chain reaction with hybridization-fluorescent detection in real time using a set of original oligonucleotides SEQ ID NO 1, SEQ ID NO 2, SEQ ID NO 3.
EFFECT: possibility of identifying genes of resistance of aadA group to aminoglycosides from samples of biological material of animals and objects of their maintenance, food raw materials, food products, without a step for isolating bacterial isolates, as well as pure bacterial cultures with high specificity and sensitivity.
1 cl, 1 dwg, 2 tbl, 2 ex
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