FIELD: medicine; pharmaceutics.
SUBSTANCE: group of inventions relates to pharmaceutical industry, namely to a method for producing an extract, and to agents based on such an extract for reducing an increase in blood glucose. Method of extraction from plants includes the following stages: stage 1): obtaining a crude extraction solution from plants by using vegetable raw materials with the addition of water, alcoholic water or acidic water and extraction by boiling in a vessel with a reflux condenser, method of boiling or using ultrasonic extraction, and concentration of crude vegetable extraction solution; step 2): separation of the concentrated crude extraction solution using a cation-exchange resin and an anion-exchange resin to obtain a collected solution, wherein: cation-exchange resin is one or a combination of several selected from strongly acidic cation-exchange resin, weakly acidic cation-exchange resin and strongly alkaline cation-exchange resin of quaternary ammonium type; amounts of cation-exchange resin and plant material used have a weight ratio in range of 1:1 to 1:30; eluent for cation-exchange resin is a salt solution containing cations, or an alkaline solution containing cations, wherein the cations in the eluent have a concentration in range of 0.04 to 5 mol/l, wherein the collection from the cation-exchange resin is started, when the pH of effluent exceeds 7, or the initial collection point of the effluent is determined in accordance with the properties of the separated components using a precipitation reaction, and the collection from the cation-exchange resin is stopped when the volume of the collected solution reaches 0.1–10 times the weight of the plant material; weight of eluent used in separation with the help of cation-exchange resin is 0.1–30 times the weight of plant material, anion-exchange resin is one or a combination of several, selected from strongly alkaline anion-exchange resin and weakly alkaline anion-exchange resin; and the amount of the anion-exchange resin and the vegetal raw material used have a weight ratio in range of 1:1 to 1:80; collection from anion-exchange resin is stopped, when the volume of the collected solution reaches 0.05–10 times the weight of the plant material; step 3): concentrating the collected solution obtained in step 2); step 4): precipitating the concentrated solution obtained in step 3) with an alcohol using ethanol as the alcohol, wherein the ethanol used for the alcohol precipitation, and vegetal raw materials have a weight ratio in range of 1:4 to 1:600; and optionally, step 5): concentration and drying, wherein the plant is a Morus plant, wherein the plant extract obtained by the extraction method from plants contains each component in the following weight ratios with respect to the total weight of the plant extract: from 30% to 99% alkaloids; from 0.2% to 35% of polysaccharides; from 0.05% to 2% of flavones; from 0% to 25% of amino acids and from 0% to 20% of other components; wherein the content of heavy metals in the plant extract is not more than 10 ppm. Herbal extract, which reduces blood glucose level, obtained using the method described above. Pharmaceutical composition for reducing an increase in the blood glucose level containing the above plant extract. Use of the herbal extract or pharmaceutical composition in the preparation of a hypoglycemic drug. Medical product for reducing an increase in the blood glucose level containing a herbal extract. Use of the herbal extract when preparing a medical product for reducing an increase in the blood glucose level.
EFFECT: above-described method of producing the extract enables to effectively reduce the content of heavy metals in plant extracts, while significantly reducing the amount of ethanol used in the extraction method, thereby improving the quality of the product while reducing production costs and increasing the efficiency and safety of industrial production to a certain extent.
11 cl, 1 dwg, 12 tbl, 14 ex
