FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a method for genotyping a polymorphic locus rs9636867 (A>G) of the IFNAR2 gene in humans. Said method is carried out by real-time PCR using allele-specific fluorescent probes. Identification of allelic variants of rs9636867 (A>G) of the IFNAR2 gene is carried out using forward and reverse primers with sequences SEQ ID NO: 1 and 2, respectively, as well as rs9636867-A-allele-specific fluorescent-labelled probe and rs9636867-G-allele-specific fluorescent-labelled probe with sequences SEQ ID NO: 3 and 4, respectively.
EFFECT: present invention provides a fast, cheap and easily reproducible method for identifying the rs9636867 (A>G) polymorphism of the IFNAR2 gene.
1 cl, 1 dwg
Authors
Dates
2024-06-03—Published
2024-04-02—Filed