FIELD: biotechnology, molecular genetic diagnostics and medicine.
SUBSTANCE: invention can be used in determining the hereditary predisposition to the development of diseases associated with carriage of rs11666524 (G>A) polymorphic variant of C19orf53 gene. The following is proposed: a method of genotyping of rs11666524 (G>A) polymorphic locus of C19orf53 gene in humans by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5′-GCCCCTATTTTGCCCTTAGA-3′ and reverse 5′-GGCTTCTGTGAGAGGACCAG-3′) and probes with fluorophores (G-allele-specific fluorescently labeled probe 5′-(FAM)TCCCACCGGGACCAGTGC(RTQ1)-3′ and A-allele-specific fluorescently labeled probe 5′-(ROX) TCCCACCAGGACCAGTGC(BHQ2)-3′) in an amplifier with the fluorescence detection.
EFFECT: expanding the range of methods of genotyping polymorphic variants of C19orf53 gene, obtaining a simple and accurate method.
1 cl, 1 dwg
