BINDING MOLECULES INDUCING CELLULAR ACTIVATION Russian patent published in 2022 - IPC C07K16/28 C07K16/46 C12N15/63 A61K39/395 A61P35/00 

FIELD: biotechnology.

SUBSTANCE: proposed is a binding molecule containing a first, a second, a third, and a fourth polypeptides. The first polypeptide and the second polypeptide comprise a light antibody chain. The third polypeptide comprises a first variable heavy chain region (VH), a first constant heavy chain region (CH1), and a second VH region. The fourth polypeptide comprises a third VH region, a second CH1 region, and a variable light chain region (VL). The first polypeptide together with the first VH region and the first CH1 region of the third polypeptide form a first antigen-binding region Fab. The second polypeptide together with the third VH region and the second CH1 region of the fourth polypeptide form a second antigen-binding Fab region. The second VH region of the third polypeptide and the VL region of the fourth polypeptide form a Fv region binding to CD3. The first and second Fab regions are connected with the Fv by a flexible peptide region containing a hinge antibody site. The first and second Fab regions bind to the same antigen. The invention also relates to a method for producing said molecule and to a pharmaceutical composition containing said molecule.

EFFECT: invention can be used in immunotherapy of cancer.

18 cl, 73 dwg, 14 tbl, 7 ex