FIELD: biotechnology.
SUBSTANCE: described is a method for indirect determination of concentration of ribonucleoprotein of industrial strain "RICH" of coronavirus infection agent in raw material for cultural vaccines according to the quantitative assessment cycle for the target area of the coronavirus M-gene.
EFFECT: main advantages of the proposed invention are reduction of time for analysis of industrial raw materials for the preparation of culture vaccines against coronavirus infection in dogs by determining the concentration of ribonucleoprotein of the virus up to 2.5 hours, which is 19 times faster than the prototype; elimination of contamination probability; high analytical and diagnostic sensitivity and specificity of the analysis; elimination of subjectivity factor of obtained results due to availability of instrumentation for detection of results; increased reliability of the analysis due to the establishment of the relationship between the concentration of the ribonucleoprotein of the strain "RICH" of the canine coronavirus (CRNP CCoV-RICH) and the cycle of quantitative assessment of amplicons of the target region of the M-gene (CQ-M), presented in the form of a quadratic function with high accuracy of approximation (R2=0.9995) and amplification efficiency (E) 100.06%.
4 cl, 3 dwg, 6 tbl, 6 ex
