FIELD: biotechnology.
SUBSTANCE: described is a method for indirect determination of the concentration of ribonucleoprotein of the canine distemper agent in raw material for culture vaccines based on the data of the quantitative assessment cycle during amplification of the target region of the N-gene.
EFFECT: main advantages of the present invention are reduction of time for analysis of industrial raw material for production of culture vaccines against CDV for determination of concentration of ribonucleoprotein CDV virus to 3 hours; elimination of contamination probability; high analytical, diagnostic sensitivity and specificity of the analysis; elimination of subjectivity factor of obtained results due to use of instrumentation for detection of results; high reliability of the analysis owing to establishing the relationship between the concentration of ribonucleoprotein CDV and the cycle of quantitative assessment of amplicons of the target region of the N-gene, presented in the form of a regression function with high values of accuracy of approximation and efficiency of the amplification reaction.
3 cl, 2 dwg, 5 tbl, 6 ex
