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2 826 458

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IPC

C12Q1/68(2006-01-01)

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Application

2023128059, 2023-10-30

(24)

Start date

2023-10-30

(22)

Actual filing date

2023-10-30

(45)

Published

2024-09-11

(72)

Inventor

Doronin Maksim IgorevichGalkina Tatyana SergeevnaMikhalishin Dmitrij ValerevichChvala Ilya AleksandrovichIsakova Darya GeorgievnaBorisov Aleksej ValerevichShevchenko Maksim AleksandrovichProkhvatilova Larisa Borisovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Tsentr Okhrany Zdorovya Zhivotnykh"

METHOD OF DIFFERENTIATING VACCINE STRAIN "RICH" FROM OTHER STRAINS AND FIELD ISOLATES OF CAUSATIVE AGENT OF CORONAVIRUS INFECTION IN DOGS BY ANALYZING MAXIMUM EXTREMA OF AMPLICON MELTING TEMPERATURE CURVES USING SUPERNOVA v428 VIOLET LASER DYE Russian patent published in 2024 - IPC C12Q1/68

Abstract RU 2826458 C1

FIELD: veterinary virology.

SUBSTANCE: invention relates to molecular diagnostics. Described is a method of differentiating the vaccine strain "RICH" from other strains and field isolates of the causative agent of coronavirus infection in dogs by analyzing maximum extrema of amplicon melting temperature curves using SuperNova v428 violet laser dye.

EFFECT: main advantages of the proposed invention are the ability to carry out in a short period of time, not more than 2,5 hours, differentiation of vaccine strain "RICH" from other strains and field isolates of causative agent of coronavirus infection of dogs; developed method is characterized by 100% analytical specificity; in 95% confidence interval diagnostic sensitivity for this method is 99,17–100%, diagnostic specificity—98,50–100%, accuracy—99,46–100%.

2 cl, 3 dwg, 5 tbl, 3 ex

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RU 2 826 458 C1

Authors

Doronin Maksim Igorevich

Galkina Tatyana Sergeevna

Mikhalishin Dmitrij Valerevich

Chvala Ilya Aleksandrovich

Isakova Darya Georgievna

Borisov Aleksej Valerevich

Shevchenko Maksim Aleksandrovich

Prokhvatilova Larisa Borisovna

Dates

2024-09-11—Published

2023-10-30—Filed