FIELD: chemistry.
SUBSTANCE: invention relates to methods of purifying protein. Disclosed is a method for purifying a recombinantly produced protein of respiratory syncytial virus (RSV), involving an anion-exchange chromatography step, where: a) an applied solution obtained from the collected cell culture liquid and comprising the RSV protein is contacted with an anion-exchange chromatography medium, as a result of which the PCB protein is bound to the anion-exchange chromatography medium; b) the medium for anion-exchange chromatography is washed with at least one washing solution with a lower pH in the range of pH 3.5–5.5, where the pH of said washing solution is lower than the pH of said applied solution, as a result of which the removal of host cell proteins is improved; and c) the RSV protein is eluted from the anion-exchange chromatography medium.
EFFECT: invention provides improved removal of host cell proteins.
28 cl, 2 dwg, 3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| HETERODIMERIC FC-FUSED PROTEINS IL15/IL15Rα | 2017 |
|
RU2779938C2 |
| ANTI-LILRB1 ANTIBODY AND USES THEREOF | 2021 |
|
RU2813373C1 |
| ANTI-LILRB1 ANTIBODY AND ITS USE | 2020 |
|
RU2801535C1 |
| POLYVALENT AND POLYSPECIFIC OX40-BINDING FUSED PROTEINS | 2017 |
|
RU2773052C2 |
| ANTIBODIES BINDING TO HUMAN CANNABINOID RECEPTOR 1 (CB1) | 2015 |
|
RU2730674C2 |
| UNIVERSAL VACCINE BASED ON COMMON TUMOR NEOANTIGENS FOR THE PREVENTION AND TREATMENT OF CANCER WITH MICROSATELLITE INSTABILITY (MSI) | 2018 |
|
RU2792843C2 |
| MOLECULES TARGETING A TYPE III SECRETION SYSTEM | 2016 |
|
RU2759949C2 |
| CHIMERIC ANTIGEN RECEPTORS AND BINDING AGENTS TARGETING DLL3 | 2020 |
|
RU2822366C2 |
| CHIMERIC ANTIGEN RECEPTORS TARGETING CD70 | 2019 |
|
RU2801824C2 |
| BISPECIFIC ANTIBODIES AND METHODS FOR THEIR PRODUCTION AND USE | 2018 |
|
RU2787783C2 |
