FIELD: biotechnology; agriculture.
SUBSTANCE: disclosed is a mutant polypeptide hydroxyphenylpyruvate dioxygenase (HPPD) coding its gene and use thereof. Mutant HPPD polypeptide retains the activity of catalyzing the conversion of hydroxyphenylpyruvic acid to homogentisic acid or homogentisate, and sensitivity to herbicides-inhibitors of HPPD is lower than sensitivity to herbicides-HPPD inhibitors of initial non-mutant HPPD. At position 372, corresponding to the amino acid sequence represented by SEQ ID NO: 1, the mutant HPPD polypeptide contains the following mutations: F372A, F372G, F372V, F372P, F372S, F372T, F372C, F372M, F372Q, F372D or F372 deletion.
EFFECT: described mutant can provide plants with high tolerance to herbicides-HPPD inhibitors and can be used for cultivation of plants which are resistant to herbicides-HPPD inhibitors.
60 cl, 7 dwg, 12 tbl, 10 ex
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