PHOSPHORAMIDE AZOLE OLIGONUCLEOTIDES, METHOD FOR SYNTHESIS OF PHOSPHORAMIDE AZOLE OLIGONUCLEOTIDES AND METHOD FOR MATRIX ENZYMATIC SYNTHESIS OF DNA USING THEM Russian patent published in 2024 - IPC A61K31/712 C07D235/04 C07D263/54 C07D277/62 C07F9/24 C07H19/10 C07H19/20 C07H21/00 C12Q1/6853 

FIELD: bioorganic chemistry.

SUBSTANCE: group of inventions relates to a phosphoramide azole oligonucleotide used as a primer during matrix enzymatic DNA synthesis; to a method for synthesis thereof; as well as to a method for matrix enzymatic synthesis of DNA using said phosphoramide azole oligonucleotide as a primer. Disclosed phosphoramide azole oligonucleotide has length of 2–23 nucleotides, contains at least one phosphoramide benzoazole group, in which an amido-benzoazole residue is introduced at the phosphorus atom, and corresponds to the formula

,

where B is a nitrogenous base, R₁ and R₂ each independently represents a hydrogen atom, or a fluorescent label, or a nucleotide residue, or an oligonucleotide residue, or a nucleotide residue containing said phosphoramide benzoazole group, or an oligonucleotide residue containing said phosphoramide benzoazole group; R₃ is absent or is -H or -C1-10-alkyl; R₄ is absent or is -C1-10-alkyl; R₅ and R₆ each independently represents -H or -OCH₃; X is -N-, -O- or -S-; Z is -H.

EFFECT: group of inventions provides high specificity of detecting analyzed nucleic acid sequences while maintaining high efficiency of PCR.

6 cl, 27 dwg, 27 ex