METHOD FOR DIAGNOSING PORCINE CIRCOVIRUS DISEASE BY FLUORESCENT HYBRIDISATION IN-SITU (FISH) Russian patent published in 2024 - IPC C12Q1/68 G01N21/64 

Abstract RU 2823532 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, particularly to a diagnostic technique for porcine circovirus disease. Said method is based on fluorescent in-situ hybridisation (FISH) and involves the use of a hybridisation probe specific to a portion of a gene encoding a capsid protein of porcine circovirus type 2, wherein the object of the study is the native cryotomic sections of the lung and inguinal lymph node. Hybridisation probe used is a nucleotide sequence with a fluorescein-12-dUTP dye.

EFFECT: invention provides detecting the genetic sequence of porcine circovirus type II in virus-susceptible cells of organs (lung, inguinal lymph node) and wider range of diagnostic techniques for porcine circovirus infection.

1 cl, 4 dwg, 3 ex

Similar patents RU2823532C1

Title Year Author Number
DIAGNOSTIC TECHNIQUE FOR PORCINE VIRAL REPRODUCTIVE-RESPIRATORY SYNDROME BY FLUORESCENT HYBRIDISATION IN-SITU (FISH) 2023
  • Stafford Viktoriya Vasilevna
  • Komina Alina Konstantinovna
  • Streltsova Yana Borisovna
  • Novichkova Elena Mikhajlovna
  • Yuzhakov Anton Gennadievich
  • Krasnikov Nikita Yurevich
  • Stepanova Tatyana Valerevna
  • Lopunov Sergej Vitalevich
RU2823531C1
DIAGNOSTIC TECHNIQUE FOR SWINE CIRCOVIRUS DISEASE OF THE SECOND TYPE WITH DIRECT IMMUNOHISTOCHEMICAL ANALYSIS ON THE BASIS OF MONOCLONAL ANTIBODIES 2018
  • Stafford Viktoriya Vasilevna
  • Raev Sergej Alekseevich
  • Kostina Lyudmila Vladimirovna
  • Kozlov Aleksej Yurevich
  • Streltsova Yana Borisovna
  • Stepanova Tatyana Valerevna
  • Drozdova Elena Ivanovna
  • Zaberezhnyj Aleksej Dmitrievich
  • Gulyukin Aleksej Mikhajlovich
  • Aliper Taras Ivanovich
  • Gulyukin Mikhail Ivanovich
  • Drobin Yurij Dmitrievich
RU2695330C1
METHOD FOR DIAGNOSTICS OF CLASSIC SWINE FEVER USING FLUORESCENT IN SITU HYBRIDIZATION (FISH) 2023
  • Stafford Viktoriya Vasilevna
  • Komina Alina Konstantinovna
  • Streltsova Yana Borisovna
  • Novichkova Elena Mikhajlovna
  • Stepanova Tatyana Valerevna
  • Drozdova Elena Ivanovna
  • Gulyukin Aleksej Mikhajlovich
RU2806252C1
DIAGNOSTIC TECHNIQUE FOR PORCINE CIRCOVIRUS 2019
  • Matveeva Irina Nikolaevna
  • Bogomolova Olesya Anatolevna
  • Litenkova Irina Yurevna
  • Samujlenko Anatolij Yakovlevich
  • Ivanov Igor Viktorovich
  • Bondareva Natalya Anatolevna
  • Pukhova Nina Mikhajlovna
  • Melnik Roman Nikolaevich
  • Kryukova Elena Nikolaevna
  • Babina Ekaterina Aleksandrovna
  • Fedorova Olga Yurevna
  • Lavrov Egor Sergeevich
  • Melnik Nikolaj Vasilevich
  • Markova Evgeniya Vladimirovna
  • Majstrenko Evgeniya Semenovna
  • Emelyanov Ilya Alekseevich
  • Grin Svetlana Anatolevna
  • Chernykh Oleg Yurevich
  • Kruglov Aleksandr Aleksandrovich
  • Fedorova Natalya Vladimirovna
  • Gadzhimuradov Oskar Alikhaevich
  • Popova Vera Mikhajlovna
  • Klyukina Valentina Ivanovna
  • Fedorov Yurij Nikolaevich
  • Preobrazhenskaya Anastasiya Sergeevna
RU2697849C1
IMMUNOGENIC COMPOSITION INDUCING IMMUNE RESPONSE AGAINST PORCINE PARVOVIRUS AND ZIRCOVIRUS, VACCINE AGAINST PORCINE MULTI-SYSTEM WEAKENING SYNDROME (PMWS) (VARIANTS), KIT FOR VACCINATION AND METHOD FOR ITS IMPLEMENTATION 1999
  • Allan Gordon Mur
  • Mikhehn Brajan Martin
  • Ehllis Dzhon Albert
  • Krakovka Dzhordzh Stiven
  • Odonne Zhan-Kristof Fransis
  • Mak-Nejli Frehnsis
RU2237492C2
II TYPE PORCINE CIRCOVIRUS AND USES THEREOF 1998
  • Allan Gordon
  • Mikhan Brajan
  • Klark Ehdvard
  • Ehllis Dzhon
  • Khejns Debora
  • Khehssard Louri
  • Kharding Dzhon
  • Sharrejr Katrin Ehlizabet
  • Shappjui Zhil' Ehmil'
  • Maknejlli Fransis
RU2283862C2
CHIMERIC PORCINE CIRCOVIRUS PCV2Gen-1Rep OF AND ITS APPLICATION 2009
  • Tszjujkhan' Nikol' M.
  • Mehn Sjan-Tszin'
RU2515901C2
METHODS AND COMPOSITIONS FOR IMMUNISATION OF PIGS AGAINST PORCINE CIRCOVIRUS 2008
  • Vu Stiven Kitu
RU2493254C9
IMMUNOGENIC COMPOSITIONS FOR IMMUNIZATION OF PIGS AGAINST CIRCOVIRUS TYPE 3 AND METHODS OF PRDUCTION AND APPLICATION THEREOF 2016
  • Hause Ben
RU2744193C2
METHOD FOR DIAGNOSIS OF CLASSICAL SWINE FEVER BY INDIRECT IMMUNOHISTOCHEMICAL ANALYSIS BASED ON MONOCLONAL ANTIBODIES 2021
  • Stafford Viktoriya Vasilevna
  • Streltsova Yana Borisovna
  • Yuzhakov Anton Gennadievich
  • Kunakov Konstantin Yurevich
  • Alekseev Konstantin Petrovich
  • Drozdova Elena Ivanovna
  • Lopunov Sergej Vitalevich
RU2782275C1

RU 2 823 532 C1

Authors

Stafford Viktoriya Vasilevna

Komina Alina Konstantinovna

Streltsova Yana Borisovna

Novichkova Elena Mikhajlovna

Gulyukin Aleksej Mikhajlovich

Dates

2024-07-23Published

2023-11-08Filed