METHOD FOR CULTIVATION OF SEA MICROALGAE PORPHYRIDIUM PURPUREUM Russian patent published in 2024 - IPC C12N1/12 

FIELD: biotechnology.

SUBSTANCE: invention represents a method for cultivation of marine microalgae Porphyridium purpureum, relates to biotechnology of microalgae, namely to intensive cultivation of red marine microalgae Porphyridium purpureum, and is intended to produce its biomass enriched with red pigment B-phycoerythrin. Objective of the present invention is to modify the water base of the nutrient medium – replacement of natural sea water with fresh water – to reduce energy consumption and labour intensity of operations when producing microalgae biomass. Method involves cultivation of microalgae P. purpureum for 10 days at temperature of 26 °C, illumination 5 klx, with continuous bubbling of culture with atmospheric air through an aquarium sprayer at rate of 0.5 l⋅l^-1 culture per minute, on a modified nutrient medium based on fresh water, having the following composition: NaNO₃ – 1.2 g⋅l^-1, NaH₂PO₄×2H₂O – 0.45 g⋅l^-1, EDTA-Na₂ – 0.037 g⋅l^-1, FeC₆H₅O₇×3H₂O – 0.0265 g⋅l^-1, MnCl₂×4H₂O – 0.004 g⋅l^-1, Co(NO₃)₂×6H₂O – 0.0031 g⋅l^-1, (NH₄)₆Mo₇O₂₄×4H₂O – 0.0009 g⋅l^-1, K₂Cr₂(SO₄)₂×4H₂O – 0.0017 g⋅l^-1. Content of sea salt in water before preparation of nutrient medium is brought to concentration of 28 g/l, and the claimed invention provides the accumulation of biomass and B-FE in the culture of P. purpureum at a level not lower than when grown on a medium based on sea water.

EFFECT: invention enables to obtain Porphyridium purpureum biomass of the required quality using a simple and affordable method while maintaining a high rate of growth of the culture.

1 cl, 3 dwg, 1 tbl