METHOD OF PRODUCING PHYCOBILIPROTEINS Russian patent published in 2024 - IPC C12N1/12 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and represents a method of producing phycobiliproteins, namely natural pigments of C-phycoerythrin and C-phycocyanin, extracted from cyanobacterium Leptolyngbya cf. ectocarpi. A method of producing phycobiliproteins from cyanobacteria Leptolyngbya cf. ectocarpi provides for obtaining biomass with high content of phycoerythrin and phycocyanin by culturing cyanobacteria on a modified nutrient medium BG11 (g/l): NaNO₃ – 3.37; NaH₂PO₄ × 2H₂O – 0.065; cіtric acid – 0.006; Na₂EDTA – 0.001; FeC₆H₅O₇ – 0.006; 1 ml of a solution of trace elements of the following composition (g/l): H₃BO₃ – 2.86; CuSO₄ × 5H₂O – 0.08; ZnSO₄ × 7H₂O – 0.22; CoCl₂ × 6H₂O – 0.05; MnCl₂ × 4H₂O – 1.81; NaMoO₄ × 2H₂O – 0.4; prepared on sterile sea water for 10 days in plane-parallel photobioreactors with a working layer of 5 cm and volume of 10 l to density of 16 g of raw biomass per 1 litre of medium under 24-hour illumination of 13 klx and temperature of 34 °C with subsequent lyophilization of cyanobacteria biomass and extraction of phycobiliproteins from lyophilized biomass with distilled water at temperature of 38 °C (1:1 lyophilized cyanobacteria biomass and distilled water) and lyophilisation of phycobiliprotein extract.

EFFECT: invention enables to extract phycobiliproteins – phycoerythrin and phycocyanin – from biomass of cyanobacteria Leptolyngbya confer ectocarpi using a single-step method, which is promising for further use on an industrial scale.

3 cl, 1 ex