FIELD: biotechnology.
SUBSTANCE: invention relates to a method for reducing aflibercept variants in a clarified collection of cells cultured in a chemically defined medium (CDM), wherein said clarified collection contains aflibercept and one or more aflibercept variants having at least one oxidised amino acid residue selected from the group consisting of tryptophan, histidine, phenylalanine, tyrosine and combinations thereof. Invention also relates to a method for reducing acid forms of aflibercept in a clarified collection of a cell cultured in a chemically defined medium (CDM).
EFFECT: invention provides effective methods for producing aflibercept.
10 cl, 59 dwg, 62 tbl, 9 ex
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